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OSGEP Polyclonal antibody

OSGEP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15033-1-AP

Synonyms

GCPL1, hOSGEP, KAE1, OSGEP, OSGEP1, PRSMG1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using OSGEP Polyclonal antibody (15033-1-AP)

    WB analysis using 15033-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15033-1-AP (OSGEP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using OSGEP Polyclonal antibody (15033-1-AP)

    WB analysis of Raji using 15033-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 15033-1-AP (OSGEP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using OSGEP Polyclonal antibody (15033-1-AP)

    WB analysis of human heart using 15033-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 15033-1-AP (OSGEP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using OSGEP Polyclonal antibody (15033-1-AP)

    IHC staining of human kidney using 15033-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15033-1-AP (OSGEP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using OSGEP Polyclonal antibody (15033-1-AP)

    IHC staining of human pancreas cancer using 15033-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15033-1-AP (OSGEP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using OSGEP Polyclonal antibody (15033-1-AP)

    IHC staining of human pancreas cancer using 15033-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15033-1-AP (OSGEP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using OSGEP Polyclonal antibody (15033-1-AP)

    IHC staining of human kidney using 15033-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15033-1-AP (OSGEP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using OSGEP Polyclonal antibody (15033-1-AP)

    IHC staining of human heart using 15033-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15033-1-AP (OSGEP Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using OSGEP Polyclonal antibody (15033-1-AP)

    IHC staining of human heart using 15033-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15033-1-AP (OSGEP Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inPC-3 cells, human heart tissue, Raji cells, mouse colon tissue
Positive IHC detected inhuman kidney tissue, human heart tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15033-1-AP targets OSGEP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OSGEP fusion protein Ag4308
Full Name O-sialoglycoprotein endopeptidase
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC032310
Gene Symbol OSGEP
Gene ID (NCBI) 55644
RRIDAB_2283171
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OSGEP(O-sialoglycoprotein endopeptidase) is also named as hOSGEP, GCPL1 and belongs to the KAE1 / YgjD family. The deduced 335-amino acid OSGEP protein has a predicted molecular mass of 36.4 kDa. It is a protease whose target is O-linked sialic acid-containing glycoproteins, such as episialin(PMID:10859271). It is involved in the metabolism of glycated proteins as a glycoprotein-related chaperon protease(PMID: 20824107).

Protocols

Product Specific Protocols
WB protocol for OSGEP antibody 15033-1-APDownload protocol
IHC protocol for OSGEP antibody 15033-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

Commonality and diversity in tRNA substrate recognition in t6A biogenesis by eukaryotic KEOPSs.

Authors - Jin-Tao Wang
View Article