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OSGEPL1 Polyclonal antibody

OSGEPL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25694-1-AP

Synonyms

OSGEPL1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using OSGEPL1 Polyclonal antibody (25694-1-AP)

    WB analysis of HeLa using 25694-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using OSGEPL1 Polyclonal antibody (25694-1-AP)

    WB analysis of NIH/3T3 using 25694-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using OSGEPL1 Polyclonal antibody (25694-1-AP)

    WB analysis of MCF-7 using 25694-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using OSGEPL1 Polyclonal antibody (25694-1-AP)

    IHC staining of human colon using 25694-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using OSGEPL1 Polyclonal antibody (25694-1-AP)

    IHC staining of human colon using 25694-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using OSGEPL1 Polyclonal antibody (25694-1-AP)

    IHC staining of human prostate hyperplasia using 25694-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate hyperplasia tissue using OSGEPL1 Polyclonal antibody (25694-1-AP)

    IHC staining of human prostate hyperplasia using 25694-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 25694-1-AP (OSGEPL1 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, NIH/3T3 cells
Positive IHC detected inhuman colon tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 25694-1-AP is OSGEPL1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OSGEPL1 fusion protein Ag22204
Full Name O-sialoglycoprotein endopeptidase-like 1
Calculated Molecular Weight 414 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC011904
Gene Symbol OSGEPL1
Gene ID (NCBI) 64172
RRIDAB_2880198
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for OSGEPL1 antibody 25694-1-APDownload protocol
IHC protocol for OSGEPL1 antibody 25694-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

CO2-sensitive tRNA modification associated with human mitochondrial disease.

Authors - Huan Lin
View Article