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- KD/KO Validated
PAIP1 Polyclonal antibody
PAIP1 Polyclonal Antibody for WB, IP, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, monkey
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 10675-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, BxPC-3 cells, COS-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IF detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 5 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
Product Information
10675-1-AP targets PAIP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, monkey samples.
| Tested Reactivity | human, monkey |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PAIP1 fusion protein Ag1070 |
| Full Name | poly(A) binding protein interacting protein 1 |
| Calculated Molecular Weight | 70 kDa |
| Observed Molecular Weight | 60 kDa, 51 kDa, 40 kDa |
| GenBank Accession Number | BC005295 |
| Gene Symbol | PAIP1 |
| Gene ID (NCBI) | 10605 |
| RRID | AB_2160391 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
In initiation of translation in eukaryotes, binding of the small ribosomal subunit to mRNA requires recognition of the 5-prime cap structure by the cap-binding complex eIF4F. eIF4F consists of eIF4E, eIF4A, and eIF4G. Translation initiation is further regulated by the mRNA 3-prime poly(A) tail and the poly(A)-binding protein (PABC1). PAIP1 interacts with PABC1 and some eIF4 complexes [PMID:9548260]. It is a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs, and associates with EIF4A and PABPC1 may potentiate contacts between mRNA termini [PMID:11051545].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PAIP1 antibody 10675-1-AP | Download protocol |
| IF protocol for PAIP1 antibody 10675-1-AP | Download protocol |
| IP protocol for PAIP1 antibody 10675-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem J Biological Insights into the Expression of Translation Initiation Factors from Recombinant CHOK1SV Cell Lines and their Relationship to Enhanced Productivity. | ||
Biol Reprod Murine PAIP1 stimulates translation of spermiogenic mRNAs stored by YBX2 via its interaction with YBX2. | ||
Hum Pathol Paip1 predicts poor prognosis and promotes tumor progression through AKT/GSK-3β pathway in lung adenocarcinoma.
| ||
Exp Cell Res Role of Paip1 on angiogenesis and invasion in pancreatic cancer.
| ||
Cancer Res Treat Paip1 Indicated Poor Prognosis in Cervical Cancer and Promoted Cervical Carcinogenesis.
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