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LYPLA3 Polyclonal antibody

LYPLA3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10863-2-AP

Synonyms

1 O acylceramide synthase, ACS, Group XV phospholipase A2, GXVPLA2, LCAT like lysophospholipase, LLPL, LPLA2, LYPLA3, Lysophospholipase 3, Lysosomal phospholipase A2, phospholipase A2, group XV, PLA2G15

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse spleen tissue using LYPLA3 Polyclonal antibody (10863-2-AP)

    WB analysis of mouse spleen using 10863-2-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10863-2-AP (LYPLA3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using LYPLA3 Polyclonal antibody (10863-2-AP)

    WB analysis of mouse kidney using 10863-2-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10863-2-AP (LYPLA3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LYPLA3 Polyclonal antibody (10863-2-AP)

    IHC staining of human liver cancer using 10863-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10863-2-AP (LYPLA3 antibody) at dilution of 1:200 (under 40x lens)..

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LYPLA3 Polyclonal antibody (10863-2-AP)

    IHC staining of human liver cancer using 10863-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10863-2-AP (LYPLA3 antibody) at dilution of 1:200 (under 10x lens)..

Tested Applications

Positive WB detected inmouse spleen tissue, mouse kidney tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10863-2-AP targets LYPLA3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LYPLA3 fusion protein Ag1304
Full Name phospholipase A2, group XV
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 42-57 kDa
GenBank Accession NumberBC011640
Gene Symbol PLA2G15
Gene ID (NCBI) 23659
RRIDAB_2283832
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLA2G15 is also named as LYPLA3 and belongs to the AB hydrolase superfamily. It has transacylase and calcium-independent phospholipase A2 activity. PLA2G15 catalyzes the formation of 1-O-acyl-N-acetylsphingosine and the concomitant release of a lyso-phospholipid. It can be detected as 42-47 kDa and 57 kDa due to the N-glycosylated modification (PMID:11790796).

Protocols

Product Specific Protocols
WB protocol for LYPLA3 antibody 10863-2-APDownload protocol
IHC protocol for LYPLA3 antibody 10863-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols