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PLA2G4B Polyclonal antibody

PLA2G4B Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 55074-1-AP

Synonyms

cPLA2 beta, HsT16992, Phospholipase A2 group IVB, PLA2G4B

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using PLA2G4B Polyclonal antibody (55074-1-AP)

    WB analysis of human plasma using 55074-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 55074-1-AP (PLA2G4B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PLA2G4B Polyclonal antibody (55074-1-AP)

    IHC staining of human gliomas using 55074-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55074-1-AP (PLA2G4B Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PLA2G4B Polyclonal antibody (55074-1-AP)

    IHC staining of human gliomas using 55074-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55074-1-AP (PLA2G4B Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55074-1-AP targets PLA2G4B in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name phospholipase A2, group IVB (cytosolic)
Calculated Molecular Weight 88 kDa
Observed Molecular Weight ~88 kDa
GenBank Accession NumberNM_001114633
Gene Symbol PLA2G4B
Gene ID (NCBI) 100137049
RRIDAB_2881261
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLA2G4B, also named as cPLA2-beta, is a member of the cytosolic phospholipase A2 protein family. It is calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position with a preference for arachidonoyl phospholipids. PLA2G4B has five isoforms with MW 88 kDa, 100 kDa, 55 kDa and 114 kDa.

Protocols

Product Specific Protocols
WB protocol for PLA2G4B antibody 55074-1-APDownload protocol
IHC protocol for PLA2G4B antibody 55074-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols