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POLR1E Polyclonal antibody

POLR1E Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 16145-1-AP

Synonyms

PAF53, POLR1E, PRAF1, RNA polymerase I subunit A49, RP11 405L18.3



Tested Applications

Positive WB detected inHeLa cells, human brain tissue, Jurkat cells, MCF-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16145-1-AP targets POLR1E in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR1E fusion protein Ag9269
Full Name polymerase (RNA) I polypeptide E, 53kDa
Calculated Molecular Weight481aa,54 kDa; 419aa,47 kDa
Observed Molecular Weight 47-53 kDa
GenBank Accession NumberBC014331
Gene Symbol POLR1E
Gene ID (NCBI) 64425
RRIDAB_2167341
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for POLR1E antibody 16145-1-APDownload protocol
IHC protocol for POLR1E antibody 16145-1-APDownload protocol
IP protocol for POLR1E antibody 16145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ChIP

Science

lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription.

Authors - Man Wu
humanWB

J Biol Chem

Identification of an E3 ligase that targets the catalytic subunit of RNA polymerase I upon transcription stress

Authors - Stephanie Pitts
WB

J Biol Chem

Conditional depletion of the RNA polymerase I subunit PAF53 reveals that it is essential for mitosis and enables identification of functional domains.

Authors - Rachel McNamar
humanWB

Cancer Invest

Quinacrine Depletes BCR-ABL and Suppresses Ph-Positive Leukemia Cells

Authors - Hu Lei
humanIF

PLoS One

Expression of RNA polymerase I catalytic core is influenced by RPA12

Authors - Brittany L Ford
humanIF

Mol Biol Cell

Nucleoli and the nucleoli-centromere association are dynamic during normal development and in cancer

Authors - Aaron Rodrigues