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POLR3D Polyclonal antibody

POLR3D Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 15339-1-AP

Synonyms

BN51, BN51T, POLR3D, Protein BN51, RNA polymerase III subunit C4, RPC4, RPC53, RPC53 homolog, TSBN51

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using POLR3D Polyclonal antibody (15339-1-AP)

    WB analysis of HeLa using 15339-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15339-1-AP (POLR3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using POLR3D Polyclonal antibody (15339-1-AP)

    WB analysis using 15339-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15339-1-AP (POLR3D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using POLR3D Polyclonal antibody (15339-1-AP)

    WB analysis of mouse brain using 15339-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15339-1-AP (POLR3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using POLR3D Polyclonal antibody (15339-1-AP)

    WB analysis of mouse testis using 15339-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15339-1-AP (POLR3D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using POLR3D Polyclonal antibody (15339-1-AP)

    IP experiment of HeLa using 15339-1-AP

    IP result of anti-POLR3D (IP:15339-1-AP, 4ug; Detection:15339-1-AP 1:300) with HeLa cells lysate 2600 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using POLR3D Polyclonal antibody (15339-1-AP)

    IF Staining of HeLa using 15339-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15339-1-AP (POLR3D antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, mouse testis tissue, HeLa cells, rat brain tissue, rat testis tissue
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15339-1-AP targets POLR3D in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR3D fusion protein Ag7542
Full Name polymerase (RNA) III (DNA directed) polypeptide D, 44kDa
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC004484
Gene Symbol POLR3D
Gene ID (NCBI) 661
RRIDAB_2168013
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Polymerase (RNA) III (DNA directed) polypeptide D(POLR3D) plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses and acts as nuclear and cytosolic DNA sensor involved in innate immune response. POLR3D can sense non-self dsDNA that serves as template for transcription into dsRNA.

Protocols

Product Specific Protocols
WB protocol for POLR3D antibody 15339-1-APDownload protocol
IF protocol for POLR3D antibody 15339-1-APDownload protocol
IP protocol for POLR3D antibody 15339-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

FEBS J

Stimulation of Pol III-dependent 5S rRNA and U6 snRNA gene expression by AP-1 transcription factors.

Authors - Richa Ahuja
View Article