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PPM1G Polyclonal antibody

Name: PPM1G Polyclonal antibody
Brand: Proteintech
SKU: 15532-1-AP

PPM1G Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15532-1-AP

Synonyms

PP2C gamma, PP2CG, PP2CGAMMA, PPM1C, PPM1G, PPP2CG, Protein phosphatase 1C, Protein phosphatase 1G

Validation Data Gallery

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AllWBIHCIF

WB analysis using 15532-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15532-1-AP (PPM1G antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 15532-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 15532-1-AP (PPM1G antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 15532-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 15532-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 15532-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 15532-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 15532-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 15532-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 15532-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 15532-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 15532-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skin using 15532-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 15532-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human brain using 15532-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 15532-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human liver using 15532-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 15532-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human heart using 15532-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 15532-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human kidney using 15532-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).
IF Staining of MCF-7 using 15532-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPM1G antibody (15532-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of MCF-7 using 15532-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPM1G antibody (15532-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 15532-1-AP

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Tested Applications

Positive WB detected in	HEK-293T cells, mouse lung tissue, Jurkat cells, MCF-7 cells
Positive IHC detected in	mouse kidney tissue, mouse testis tissue, human heart tissue, human kidney tissue, human skin tissue, human placenta tissue, human brain tissue, mouse spleen tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 1 publications below

Product Information

15532-1-AP targets PPM1G in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PPM1G fusion protein Ag7751
Full Name	protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
Calculated Molecular Weight	59 kDa
Observed Molecular Weight	70 kDa
GenBank Accession Number	BC000057
Gene Symbol	PPM1G
Gene ID (NCBI)	5496
RRID	AB_2169934
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPM1G, also known as PP2Cγ and PP2CG, is an important member of the PP2C family of serine/threonine protein phosphatases and has an important role in controlling cell cycle progression. PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasio(PMID: 33219031). In addition, PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3(PMID: 34290239).

Protocols

Product Specific Protocols
WB protocol for PPM1G antibody 15532-1-AP	Download protocol
IHC protocol for PPM1G antibody 15532-1-AP	Download protocol
IF protocol for PPM1G antibody 15532-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Br J Pharmacol Costunolide represses hepatic fibrosis through WWP2-mediated Notch3 degradation. Authors - Mao-Xu Ge View Article
human	WB	J Oncol Identifying Potential Tumor Antigens and Antigens-Related Subtypes in Hepatocellular Carcinoma for mRNA Vaccine Development Authors - Weiran Liao View Article
human	WB,IHC	Cell Death Dis PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3. Authors - Dawei Chen KD Validated View Article

PPM1G Polyclonal antibody

PPM1G Polyclonal Antibody for IF, IHC, WB, ELISA

Validation Data Gallery

WB analysis using 15532-1-AP

WB analysis of mouse lung using 15532-1-AP

IHC staining of mouse testis using 15532-1-AP

IHC staining of mouse kidney using 15532-1-AP

IHC staining of mouse kidney using 15532-1-AP

IHC staining of mouse testis using 15532-1-AP

IHC staining of human placenta using 15532-1-AP

IHC staining of human placenta using 15532-1-AP

IHC staining of mouse spleen using 15532-1-AP

IHC staining of mouse spleen using 15532-1-AP

IHC staining of human kidney using 15532-1-AP

IHC staining of human skin using 15532-1-AP

IHC staining of human skin using 15532-1-AP

IHC staining of human brain using 15532-1-AP

IHC staining of human brain using 15532-1-AP

IHC staining of human liver using 15532-1-AP

IHC staining of human liver using 15532-1-AP

IHC staining of human heart using 15532-1-AP