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PPOX Polyclonal antibody

PPOX Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14870-1-AP

Synonyms

PPO, PPOX, protoporphyrinogen oxidase, V290M, VP

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using PPOX Polyclonal antibody (14870-1-AP)

    WB analysis of human placenta using 14870-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 14870-1-AP (PPOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PPOX Polyclonal antibody (14870-1-AP)

    IHC staining of human kidney using 14870-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14870-1-AP (PPOX antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PPOX Polyclonal antibody (14870-1-AP)

    IHC staining of human kidney using 14870-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14870-1-AP (PPOX antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

14870-1-AP targets PPOX in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPOX fusion protein Ag6541
Full Name protoporphyrinogen oxidase
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC002357
Gene Symbol PPOX
Gene ID (NCBI) 5498
RRIDAB_10597704
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PPOX antibody 14870-1-APDownload protocol
IHC protocol for PPOX antibody 14870-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Med (Lausanne)

Heme Biosynthesis Factors and 5-ALA Induced Fluorescence: Analysis of mRNA and Protein Expression in Fluorescing and Non-fluorescing Gliomas.

Authors - Mario Mischkulnig
View Article