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PRAP1 Polyclonal antibody

PRAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11932-1-AP

Synonyms

PRAP1, PRO1195, proline rich acidic protein 1, RP11 122K13.6, UPA

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using PRAP1 Polyclonal antibody (11932-1-AP)

    WB analysis of L02 using 11932-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 11932-1-AP (PRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using PRAP1 Polyclonal antibody (11932-1-AP)

    WB analysis of mouse pancreas using 11932-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11932-1-AP (PRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using PRAP1 Polyclonal antibody (11932-1-AP)

    WB analysis of L02 using 11932-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 11932-1-AP (PRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using PRAP1 Polyclonal antibody (11932-1-AP)

    IHC staining of human liver using 11932-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PRAP1 Polyclonal antibody (11932-1-AP)

    IHC staining of human liver using 11932-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PRAP1 Polyclonal antibody (11932-1-AP)

    IHC staining of human kidney using 11932-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using PRAP1 Polyclonal antibody (11932-1-AP)

    IHC staining of mouse small intestine using 11932-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 11932-1-AP (PRAP1 Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using PRAP1 Polyclonal antibody (11932-1-AP)

    IHC staining of mouse small intestine using 11932-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 11932-1-AP (PRAP1 Antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inL02 cells, mouse pancreas tissue
Positive IHC detected inhuman liver tissue, human kidney tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11932-1-AP targets PRAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRAP1 fusion protein Ag2526
Full Name proline-rich acidic protein 1
Calculated Molecular Weight 151 aa, 17 kDa
Observed Molecular Weight 16-20 kDa
GenBank Accession NumberBC029447
Gene Symbol PRAP1
Gene ID (NCBI) 118471
RRIDAB_10951856
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proline rich acidic protein 1 (PRAP1) is also named as PRO1195 or UPA. It may play an important role in maintaining normal growth homeostasis in epithelial cells. Mitotic arrest deficient 1 (MAD1) is a key component in mitotic checkpoint signalling. Recent study identified PRAP1 as a novel MAD1 binding partner using yeast-two hybrid screening (PMID: 24374861). PRAP1 has four isoforms with MW 16-20 kDa and 10 kDa.

Protocols

Product Specific Protocols
WB protocol for PRAP1 antibody 11932-1-APDownload protocol
IHC protocol for PRAP1 antibody 11932-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,WB

Adv Sci (Weinh)

Adipocyte-Derived Exosomal MTTP Suppresses Ferroptosis and Promotes Chemoresistance in Colorectal Cancer

Authors - Qiumo Zhang
  • KD Validated
View Article