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  • Featured Product
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PRKACA Monoclonal antibody

PRKACA Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4G10D7

Cat no : 67491-1-Ig

Synonyms

PKA C alpha, PKACA, PRKACA

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PRKACA Monoclonal antibody (67491-1-Ig)

    WB analysis of HeLa using 67491-1-Ig

    WB result of PRKACA antibody (67491-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRKACA transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using PRKACA Monoclonal antibody (67491-1-Ig)

    WB analysis using 67491-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67491-1-Ig (PRKACA antibody) at dilution of 1:2300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRKACA Monoclonal antibody (67491-1-Ig)

    IHC staining of human breast cancer using 67491-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67491-1-Ig (PRKACA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRKACA Monoclonal antibody (67491-1-Ig)

    IHC staining of human breast cancer using 67491-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67491-1-Ig (PRKACA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PRKACA Monoclonal antibody (67491-1-Ig)

    IHC staining of human prostate cancer using 67491-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67491-1-Ig (PRKACA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PRKACA Monoclonal antibody (67491-1-Ig)

    IHC staining of human prostate cancer using 67491-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67491-1-Ig (PRKACA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, LNCaP cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67491-1-Ig targets PRKACA in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PRKACA fusion protein Ag26400
Full Name protein kinase, cAMP-dependent, catalytic, alpha
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 38-43 kDa
GenBank Accession NumberBC039846
Gene Symbol PRKACA
Gene ID (NCBI) 5566
RRIDAB_2882716
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRKACA, also named as PKACA and PKA C-alpha, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It phosphorylates a large number of substrates in the cytoplasm and the nucleus.

Protocols

Product Specific Protocols
WB protocol for PRKACA antibody 67491-1-IgDownload protocol
IHC protocol for PRKACA antibody 67491-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols