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PRUNE Polyclonal antibody

PRUNE Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18537-1-AP

Synonyms

DRES 17, DRES17, hPrune, HTCD37, Protein prune homolog, PRUNE, prune homolog (Drosophila)

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRUNE Polyclonal antibody (18537-1-AP)

    WB analysis using 18537-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18537-1-AP (PRUNE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PRUNE Polyclonal antibody (18537-1-AP)

    WB analysis of HepG2 using 18537-1-AP

    WB result of PRUNE antibody (18537-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRUNE transfected HepG2 cells.

  • Immunoprecipitation (IP) experiment of HepG2 cells using PRUNE Polyclonal antibody (18537-1-AP)

    IP experiment of HepG2 using 18537-1-AP

    IP result of anti-PRUNE (IP:18537-1-AP, 3ug; Detection:18537-1-AP 1:500) with HepG2 cells lysate 1720ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PRUNE Polyclonal antibody (18537-1-AP)

    IHC staining of human liver cancer using 18537-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18537-1-AP (PRUNE antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PRUNE Polyclonal antibody (18537-1-AP)

    IHC staining of human liver cancer using 18537-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18537-1-AP (PRUNE antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRUNE Polyclonal antibody (18537-1-AP)

    IF Staining of HepG2 using 18537-1-AP

    Immunofluorescent analysis of HepG2 cells, using PRUNE antibody 18537-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inA431 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18537-1-AP targets PRUNE in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRUNE fusion protein Ag13275
Full Name prune homolog (Drosophila)
Calculated Molecular Weight 453 aa, 50 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC025304
Gene Symbol PRUNE
Gene ID (NCBI) 58497
RRIDAB_2172338
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PRUNE antibody 18537-1-APDownload protocol
IHC protocol for PRUNE antibody 18537-1-APDownload protocol
IF protocol for PRUNE antibody 18537-1-APDownload protocol
IP protocol for PRUNE antibody 18537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols