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PSMA4 Polyclonal antibody

PSMA4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11943-2-AP

Synonyms

20S proteasome, HC9, HsT17706, Macropain subunit C9, Proteasome component C9, Proteasome subunit L, PSC9, PSMA4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMA4 Polyclonal antibody (11943-2-AP)

    WB analysis using 11943-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11943-2-AP (PSMA4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using PSMA4 Polyclonal antibody (11943-2-AP)

    WB analysis of mouse kidney using 11943-2-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11943-2-AP (PSMA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using PSMA4 Polyclonal antibody (11943-2-AP)

    WB analysis of mouse spleen using 11943-2-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11943-2-AP (PSMA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of BxPC-3 cells using PSMA4 Polyclonal antibody (11943-2-AP)

    IP experiment of BxPC-3 using 11943-2-AP

    IP result of anti-PSMA4 (IP:11943-2-AP, 4ug; Detection:11943-2-AP 1:500) with BxPC-3 cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PSMA4 Polyclonal antibody (11943-2-AP)

    IHC staining of mouse liver using 11943-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11943-2-AP (PSMA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PSMA4 Polyclonal antibody (11943-2-AP)

    IHC staining of mouse liver using 11943-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11943-2-AP (PSMA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PSMA4 Polyclonal antibody (11943-2-AP)

    IHC staining of human pancreas cancer using 11943-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11943-2-AP (PSMA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PSMA4 Polyclonal antibody (11943-2-AP)

    IHC staining of human pancreas cancer using 11943-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11943-2-AP (PSMA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMA4 Polyclonal antibody (11943-2-AP)

    IF Staining of HeLa using 11943-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11943-2-AP (PSMA4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inBxPC-3 cells, mouse kidney tissue, mouse spleen tissue, K-562 cells, U-937 cells
Positive IP detected inBxPC-3 cells
Positive IHC detected inhuman pancreas cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11943-2-AP targets PSMA4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMA4 fusion protein Ag2542
Full Name proteasome (prosome, macropain) subunit, alpha type, 4
Calculated Molecular Weight 237 aa, 27 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC056249
Gene Symbol PSMA4
Gene ID (NCBI) 5685
RRIDAB_874213
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PSMA4 antibody 11943-2-APDownload protocol
IHC protocol for PSMA4 antibody 11943-2-APDownload protocol
IF protocol for PSMA4 antibody 11943-2-APDownload protocol
IP protocol for PSMA4 antibody 11943-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

J Biol Chem

PITHD1 is a proteasome-interacting protein essential for male fertilization.

Authors - Hiroyuki Kondo
View Article
mouseIHC

Sci Rep

Proteasome inhibition by bortezomib parallels a reduction in head and neck cancer cells growth, and an increase in tumor-infiltrating immune cells.

Authors - Monica Benvenuto
View Article
humanWB

PLoS One

Inactive USP14 and inactive UCHL5 cause accumulation of distinct ubiquitinated proteins in mammalian cells.

Authors - Jayashree Chadchankar
View Article
humanWB

Cell Death Differ

Quantitative reactive cysteinome profiling reveals a functional link between ferroptosis and proteasome-mediated degradation

Authors - Yankun Wang
View Article