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PSMC6 Polyclonal antibody

PSMC6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15839-1-AP

Synonyms

CADP44, p42, P44, Proteasome subunit p42, PSMC6, Rpt4, SUG2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMC6 Polyclonal antibody (15839-1-AP)

    WB analysis using 15839-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PSMC6 Polyclonal antibody (15839-1-AP)

    WB analysis of mouse brain using 15839-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using PSMC6 Polyclonal antibody (15839-1-AP)

    WB analysis of rat liver using 15839-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PSMC6 Polyclonal antibody (15839-1-AP)

    WB analysis of human brain using 15839-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using PSMC6 Polyclonal antibody (15839-1-AP)

    WB analysis of human testis using 15839-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSMC6 Polyclonal antibody (15839-1-AP)

    IHC staining of human liver cancer using 15839-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15839-1-AP (PSMC6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSMC6 Polyclonal antibody (15839-1-AP)

    IHC staining of human liver cancer using 15839-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15839-1-AP (PSMC6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PSMC6 Polyclonal antibody (15839-1-AP)

    IHC staining of mouse brain using 15839-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15839-1-AP (PSMC6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PSMC6 Polyclonal antibody (15839-1-AP)

    IHC staining of mouse brain using 15839-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15839-1-AP (PSMC6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, human testis tissue, rat liver tissue, mouse liver tissue, rat brain tissue
Positive IHC detected inhuman liver cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15839-1-AP targets PSMC6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMC6 fusion protein Ag8589
Full Name proteasome (prosome, macropain) 26S subunit, ATPase, 6
Calculated Molecular Weight 389 aa, 44 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC005390
Gene Symbol PSMC6
Gene ID (NCBI) 5706
RRIDAB_2237797
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMC6(proteasome (prosome, macropain) 26S subunit, ATPase, 6) is also named as CADP44, MGC12520, P44, SUG2, p42 and belongs to the AAA ATPase family. It is a component of the 26S protease, which is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. The gene encodes a 44 kDa PSMC6.

Protocols

Product Specific Protocols
WB protocol for PSMC6 antibody 15839-1-APDownload protocol
IHC protocol for PSMC6 antibody 15839-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Biomed Sci

Znf179 E3 ligase-mediated TDP-43 polyubiquitination is involved in TDP-43- ubiquitinated inclusions (UBI) (+)-related neurodegenerative pathology.

Authors - Yi-Chao Lee
View Article
mouseIHC

FASEB J

Glucose-induced decrease of cystathionine β-synthase mediates renal injuries.

Authors - Yanting Yu
View Article
ratWB

Cardiovasc Drugs Ther

Quercetin Prevents In Vivo and In Vitro Myocardial Hypertrophy Through the Proteasome-GSK-3 Pathway.

Authors - Kuixiang Chen
View Article
mouse,ratWB

Acta Pharmacol Sin

Zonisamide attenuates pressure overload-induced myocardial hypertrophy in mice through proteasome inhibition

Authors - Qian Wu
View Article