- Featured Product
- KD/KO Validated
PSME4 Polyclonal antibody
PSME4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 18799-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
18799-1-AP targets PSME4 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | proteasome (prosome, macropain) activator subunit 4 |
| Calculated Molecular Weight | 211 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC113668 |
| Gene Symbol | PSME4 |
| Gene ID (NCBI) | 23198 |
| RRID | AB_10598020 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PSME4, like PSME1, 2, and 3, encodes proteasome activator complex subunit 4 also named proteasome activator 200 kDa (PA200). PA200 activates proteasomal cleavage of peptides in an energy-independent manner. After exposure of cells to ionizing radiation, PA200 forms nuclear foci and enhances proteasome activity independent of the stage of cell cycle arrest. PA200 also may be involved in spermatogenesis. The antibody is expected to recognize all the 4 isoforms of PSME4 but appears to mainly detect isoform 2 of 140-kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PSME4 antibody 18799-1-AP | Download protocol |
| IHC protocol for PSME4 antibody 18799-1-AP | Download protocol |
| IF protocol for PSME4 antibody 18799-1-AP | Download protocol |
| IP protocol for PSME4 antibody 18799-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EBioMedicine CRIP1 involves the pathogenesis of multiple myeloma via dual-regulation of proteasome and autophagy | ||
EBioMedicine Indirubin-3'-monoxime acts as proteasome inhibitor: Therapeutic application in multiple myeloma.
| ||
Int J Gen Med PSME4 Activates mTOR Signaling and Promotes the Malignant Progression of Hepatocellular Carcinoma.
|
