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PWP2 Polyclonal antibody

PWP2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 11390-1-AP

Synonyms

EHOC 17, PWP2, PWP2H

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using PWP2 Polyclonal antibody (11390-1-AP)

    WB analysis of HeLa using 11390-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11390-1-AP (PWP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using PWP2 Polyclonal antibody (11390-1-AP)

    IP experiment of HeLa using 11390-1-AP

    IP result of anti-PWP2 (IP:11390-1-AP, 4ug; Detection:11390-1-AP 1:600) with HeLa cells lysate 2400ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PWP2 Polyclonal antibody (11390-1-AP)

    IF Staining of HepG2 using 11390-1-AP

    Immunofluorescent analysis of HepG2 cells, using PWP2 antibody 11390-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11390-1-AP targets PWP2 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PWP2 fusion protein Ag1936
Full Name PWP2 periodic tryptophan protein homolog (yeast)
Calculated Molecular Weight 919 aa, 102 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC014988
Gene Symbol PWP2
Gene ID (NCBI) 5822
RRIDAB_2284709
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of PWP2 remains largely unknown. Catalog#11390-1-AP is a rabbit polyclonal antibody raised against the full-length of human PWP2. The MW of this protein is 102 kDa, and this antibody specially recognises the 102 kDa protein.

Protocols

Product Specific Protocols
WB protocol for PWP2 antibody 11390-1-APDownload protocol
IF protocol for PWP2 antibody 11390-1-APDownload protocol
IP protocol for PWP2 antibody 11390-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

Derivation and Validation of a Prognostic Model for Cancer Dependency Genes Based on CRISPR-Cas9 in Gastric Adenocarcinoma.

Authors - Wenjie Zhou
View Article