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RABEP2 Monoclonal antibody

RABEP2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A4A4

Cat no : 60125-1-Ig

Synonyms

FRA, Rabaptin 5beta, RABEP2, RABPT5B

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using RABEP2 Monoclonal antibody (60125-1-Ig)

    WB analysis of Jurkat using 60125-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 60125-1-Ig (RABEP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using RABEP2 Monoclonal antibody (60125-1-Ig)

    WB analysis of HSC-T6 using 60125-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60125-1-Ig (RABEP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using RABEP2 Monoclonal antibody (60125-1-Ig)

    WB analysis of PC-3 using 60125-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 60125-1-Ig (RABEP2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RABEP2 Monoclonal antibody (60125-1-Ig)

    IHC staining of human prostate cancer using 60125-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60125-1-Ig (RABEP2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RABEP2 Monoclonal antibody (60125-1-Ig)

    IHC staining of human prostate cancer using 60125-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60125-1-Ig (RABEP2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RABEP2 Monoclonal antibody (60125-1-Ig)

    IHC staining of human breast cancer using 60125-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60125-1-Ig (RABEP2 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RABEP2 Monoclonal antibody (60125-1-Ig)

    IHC staining of human breast cancer using 60125-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60125-1-Ig (RABEP2 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHSC-T6 cells, Jurkat cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60125-1-Ig targets RABEP2 in WB, IHC, ELISA applications and shows reactivity with human, rat, pig samples.

Tested Reactivity human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RABEP2 fusion protein Ag6197
Full Name rabaptin, RAB GTPase binding effector protein 2
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC058900
Gene Symbol RABEP2
Gene ID (NCBI) 79874
RRIDAB_2284821
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RABEP2, also named as RABPT5B, plays a role in membrane trafficking and in homotypic early endosome fusion. It has two isoforms with MW 59-63 kDa.

Protocols

Product Specific Protocols
WB protocol for RABEP2 antibody 60125-1-IgDownload protocol
IHC protocol for RABEP2 antibody 60125-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols