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- KD/KO Validated
RabGEF1 Polyclonal antibody
RabGEF1 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12735-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-3 cells |
| Positive IHC detected in | human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
12735-1-AP targets RabGEF1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RabGEF1 fusion protein Ag3442 |
| Full Name | RAB guanine nucleotide exchange factor (GEF) 1 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC015330 |
| Gene Symbol | RABGEF1 |
| Gene ID (NCBI) | 27342 |
| RRID | AB_2175788 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RabGEF1 (Rabex-5) is a guanine nucleotide exchange factor (GEF) for the endocytic pathway regulator Rab5. RabGEF1 contains a Vps9 domain, an A20-like zinc finger (ZnF) domain, and a coiled coil domain (PMID: 17341663). RabGEF1 is involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. It is also implicated in tumorigenesis and in the development of certain human cancers (PMID: 25427001).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RabGEF1 antibody 12735-1-AP | Download protocol |
| IHC protocol for RabGEF1 antibody 12735-1-AP | Download protocol |
| IF protocol for RabGEF1 antibody 12735-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Rep AP-1/σ1A and AP-1/σ1B adaptor-proteins differentially regulate neuronal early endosome maturation via the Rab5/Vps34-pathway. | ||
Exp Mol Pathol RabGEF1 functions as an oncogene in U251 glioblastoma cells and is involved in regulating AKT and Erk pathways.
| ||
Orthop Surg LncRNA TM1-3P Regulates Proliferation, Apoptosis and Inflammation of Fibroblasts in Osteoarthritis through miR-144-3p/ONECUT2 Axis | ||
Cell Rep Endosomal Dysfunction Induced by Directly Overactivating Rab5 Recapitulates Prodromal and Neurodegenerative Features of Alzheimer's Disease. | ||
J Cell Sci Vps9d1 regulates tubular endosome formation through specific activation of Rab22A |
