• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RBM25 Polyclonal antibody

RBM25 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, RIP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25297-1-AP

Synonyms

fSAP94, NET52, Protein S164, RBM25, RED120, RNA binding motif protein 25, RNA binding protein 25, RNPC7, S164

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RBM25 Polyclonal antibody (25297-1-AP)

    WB analysis using 25297-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25297-1-AP (RBM25 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using RBM25 Polyclonal antibody (25297-1-AP)

    IP experiment of HEK-293 using 25297-1-AP

    IP result of anti-RBM25 (IP:25297-1-AP, 4ug; Detection:25297-1-AP 1:1000) with HEK-293 cells lysate 1400 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RBM25 Polyclonal antibody (25297-1-AP)

    IHC staining of human breast cancer using 25297-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25297-1-AP (RBM25 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RBM25 Polyclonal antibody (25297-1-AP)

    IHC staining of human cervical cancer using 25297-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25297-1-AP (RBM25 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RBM25 Polyclonal antibody (25297-1-AP)

    IHC staining of human breast cancer using 25297-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25297-1-AP (RBM25 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RBM25 Polyclonal antibody (25297-1-AP)

    IHC staining of human cervical cancer using 25297-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25297-1-AP (RBM25 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBM25 Polyclonal antibody (25297-1-AP)

    IF Staining of HeLa using 25297-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM25 antibody (25297-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, mouse brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25297-1-AP targets RBM25 in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM25 fusion protein Ag18244
Full Name RNA binding motif protein 25
Calculated Molecular Weight 843 aa, 100 kDa
Observed Molecular Weight 110-120 kDa
GenBank Accession NumberBC113389
Gene Symbol RBM25
Gene ID (NCBI) 58517
RRIDAB_2880014
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA-binding protein 25 (RBM25), also named as Protein S164 or RNPC7, is a 843 amino acid protein, which contains one RRM domain and one PWI domain. RBM25 localizes in the nucleus and cytoplasm. RBM25 acts as a regulator of alternative pre-mRNA splicing and is involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression.

Protocols

Product Specific Protocols
WB protocol for RBM25 antibody 25297-1-APDownload protocol
IHC protocol for RBM25 antibody 25297-1-APDownload protocol
IF protocol for RBM25 antibody 25297-1-APDownload protocol
IP protocol for RBM25 antibody 25297-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseRIP

Arterioscler Thromb Vasc Biol

Super Enhancer-Associated Circular RNA-CircKrt4 Regulates Hypoxic Pulmonary Artery Endothelial Cell Dysfunction in Mice

Authors - Cui Ma
View Article
ratIP

PeerJ

RBM25 binds to and regulates alternative splicing levels of Slc38a9, Csf1, and Coro6 to affect immune and inflammatory processes in H9c2 cells

Authors - Xin Tian
View Article