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  • Featured Product
  • KD/KO Validated

RERG Polyclonal antibody

RERG Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10687-1-AP

Synonyms

RERC, RERG

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using RERG Polyclonal antibody (10687-1-AP)

    WB analysis of MCF-7 using 10687-1-AP

    WB result of RERG antibody (10687-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RERG transfected MCF-7 cells.

  • Western Blot (WB) analysis of various lysates using RERG Polyclonal antibody (10687-1-AP)

    WB analysis using 10687-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10687-1-AP (RERG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RERG Polyclonal antibody (10687-1-AP)

    WB analysis of NIH/3T3 using 10687-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10687-1-AP (RERG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using RERG Polyclonal antibody (10687-1-AP)

    WB analysis of MCF-7 using 10687-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10687-1-AP (RERG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inNIH/3T3 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10687-1-AP targets RERG in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RERG fusion protein Ag1041
Full Name RAS-like, estrogen-regulated, growth inhibitor
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23-26 kDa
GenBank Accession NumberBC007997
Gene Symbol RERG
Gene ID (NCBI) 85004
RRIDAB_2253772
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RERG (Ras-related and estrogen-regulated growth inhibitor), a gene that encodes a small GTP binding and hydrolyzing protein (GTPase) of the Ras superfamily, is a novel gene that was first reported in breast cancer. RERG gene expression is stimulated by the estrogen receptor alpha (ERalpha), and RERG gene expression is absent in ER-negative breast cancer. RERG can bind and hydrolyze GTP as a GTP/GDP molecular switch, and RERG has been shown to possess growth inhibitory activity in breast cancer cells.

Protocols

Product Specific Protocols
WB protocol for RERG antibody 10687-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Front Oncol

The NFAT3/RERG Complex in Luminal Breast Cancers Is Required to Inhibit Cell Invasion and May Be Correlated With an Absence of Axillary Lymph Nodes Colonization.

Authors - Lucie Coillard
  • KD Validated
View Article
humanIHC

Breast Cancer Res Treat

RERG (Ras-like, oestrogen-regulated, growth-inhibitor) expression in breast cancer: a marker of ER-positive luminal-like subtype.

Authors - Habashy Hany Onsy HO
View Article

Oncol Rep

ERK5‑regulated RERG expression promotes cancer progression in prostatic carcinoma.

Authors - Ying Xiong
View Article
humanWB,IHC

Oncotarget

MicroRNA-382-5p aggravates breast cancer progression by regulating the RERG/Ras/ERK signaling axis.

Authors - Jar-Yi Ho
View Article
humanIHC

Front Med (Lausanne)

Missing link between tissue specific expressing pattern of ERβ and the clinical manifestations in LGBLEL

Authors - Xujuan Zhang
View Article