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  • Featured Product
  • KD/KO Validated

REXO4 Polyclonal antibody

REXO4 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18890-1-AP

Synonyms

Exonuclease XPMC2, hPMC2, PMC2, REX4, REXO4, RNA exonuclease 4, XPMC2, XPMC2H

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using REXO4 Polyclonal antibody (18890-1-AP)

    WB analysis using 18890-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18890-1-AP (REXO4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using REXO4 Polyclonal antibody (18890-1-AP)

    WB analysis of HeLa using 18890-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18890-1-AP (REXO4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using REXO4 Polyclonal antibody (18890-1-AP)

    WB analysis of HepG2 using 18890-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18890-1-AP (REXO4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using REXO4 Polyclonal antibody (18890-1-AP)

    IHC staining of human testis using 18890-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18890-1-AP (REXO4 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using REXO4 Polyclonal antibody (18890-1-AP)

    IHC staining of human testis using 18890-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18890-1-AP (REXO4 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using REXO4 Polyclonal antibody (18890-1-AP)

    IF Staining of MCF-7 using 18890-1-AP

    Immunofluorescent analysis of MCF-7 cells, using REX4 antibody 18890-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18890-1-AP targets REXO4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen REXO4 fusion protein Ag13473
Full Name REX4, RNA exonuclease 4 homolog (S. cerevisiae)
Calculated Molecular Weight 422 aa, 47 kDa
Observed Molecular Weight 47-50 kDa
GenBank Accession NumberBC009274
Gene Symbol REX4
Gene ID (NCBI) 57109
RRIDAB_10643245
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for REXO4 antibody 18890-1-APDownload protocol
IHC protocol for REXO4 antibody 18890-1-APDownload protocol
IF protocol for REXO4 antibody 18890-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Gastrointest Oncol

REXO4 acts as a biomarker and promotes hepatocellular carcinoma progression.

Authors - Yun Ruan
  • KD Validated
View Article
humanWB

J Adv Res

PROTAC derivatization of natural products for target identification and drug discovery: Design of evodiamine-based PROTACs as novel REXO4 degraders

Authors - Shuqiang Chen
View Article