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  • Featured Product
  • KD/KO Validated

RPAP1 Polyclonal antibody

RPAP1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 15138-1-AP

Synonyms

KIAA1403, RPAP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPAP1 Polyclonal antibody (15138-1-AP)

    WB analysis using 15138-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15138-1-AP (RPAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RPAP1 Polyclonal antibody (15138-1-AP)

    WB analysis of HEK-293 using 15138-1-AP

    WB result of RPAP1 antibody (15138-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPAP1 transfected HEK-293 cells.

  • Immunoprecipitation (IP) experiment of HeLa cells using RPAP1 Polyclonal antibody (15138-1-AP)

    IP experiment of HeLa using 15138-1-AP

    IP result of anti-RPAP1 (IP:15138-1-AP, 3ug; Detection:15138-1-AP 1:800) with HeLa cells lysate 3000ug.

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15138-1-AP targets RPAP1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPAP1 fusion protein Ag7286
Full Name RNA polymerase II associated protein 1
Calculated Molecular Weight 153 kDa
Observed Molecular Weight 153 kDa
GenBank Accession NumberBC000246
Gene Symbol RPAP1
Gene ID (NCBI) 26015
RRIDAB_2301137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNA polymerase II-associated protein 1 (RPAP1) encodes a nuclear 153-kDa protein, and forms an interface between the RNA polymerase II (RNAPII) enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. RPAP1 is required for interaction of the RNA polymerase II complex with acetylated histone H3 and the subsequent DNA transcription.

Protocols

Product Specific Protocols
WB protocol for RPAP1 antibody 15138-1-APDownload protocol
IP protocol for RPAP1 antibody 15138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Rep

The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity.

Authors - Cian J Lynch
  • KD Validated
View Article
humanWB

Cell Rep

Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CLpro substrate degradome.

Authors - Isabel Pablos
View Article