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RRP36 Polyclonal antibody

RRP36 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 25440-1-AP

Synonyms

C6orf153, HSPC253, RRP36

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using RRP36 Polyclonal antibody (25440-1-AP)

    WB analysis of MCF-7 using 25440-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25440-1-AP (RRP36 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RRP36 Polyclonal antibody (25440-1-AP)

    WB analysis of HEK-293 using 25440-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25440-1-AP (RRP36 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RRP36 Polyclonal antibody (25440-1-AP)

    IF Staining of MCF-7 using 25440-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 25440-1-AP (RRP36 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25440-1-AP is RRP36 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RRP36 fusion protein Ag22014
Full Name chromosome 6 open reading frame 153
Calculated Molecular Weight 259 aa, 30 kDa
Observed Molecular Weight 30-40 kDa
GenBank Accession NumberBC011933
Gene Symbol C6orf153
Gene ID (NCBI) 88745
RRIDAB_2880082
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RRP36 antibody 25440-1-APDownload protocol
IF protocol for RRP36 antibody 25440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols