RSPO1 Polyclonal antibody
RSPO1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 25348-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human plasma, rat plasma |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IF | See 1 publications below |
Product Information
25348-1-AP targets RSPO1 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RSPO1 fusion protein Ag21858 |
| Full Name | R-spondin homolog (Xenopus laevis) |
| Calculated Molecular Weight | 263 aa, 29 kDa |
| Observed Molecular Weight | 29 kDa |
| GenBank Accession Number | BC114966 |
| Gene Symbol | RSPO1 |
| Gene ID (NCBI) | 284654 |
| RRID | AB_2880038 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RSPO1, also named R-spondin-1, Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential role in ovary determination. Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1. RSPO1 seems to mainly localize to nucleoli (PMID:17804805).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RSPO1 antibody 25348-1-AP | Download protocol |
| IHC protocol for RSPO1 antibody 25348-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Am J Hum Genet Functional analysis of the 1p34.3 risk locus implicates GNL2 in high-grade serous ovarian cancer. | ||
Front Mol Biosci A Novel Identified Long Non-coding RNA, lncRNA MEF2C-AS1, Inhibits Cervical Cancer via Regulation of miR-592/RSPO1. | ||
J Exp Med Transit-amplifying cells control R-spondins in the mouse crypt to modulate intestinal stem cell proliferation |
