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RTEL1 Polyclonal antibody

RTEL1 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 25337-1-AP

Synonyms

bK3184A7.3, C20orf41, KIAA1088, NHL, Novel helicase like, RTEL, RTEL1

Validation Data Gallery

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  • Western Blot (WB) analysis of BGC-823 cells using RTEL1 Polyclonal antibody (25337-1-AP)

    WB analysis of BGC-823 using 25337-1-AP

    BGC-823 cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RTEL1 Polyclonal antibody (25337-1-AP)

    WB analysis of HepG2 using 25337-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using RTEL1 Polyclonal antibody (25337-1-AP)

    WB analysis of mouse testis using 25337-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using RTEL1 Polyclonal antibody (25337-1-AP)

    WB analysis of C6 using 25337-1-AP

    C6 cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RTEL1 Polyclonal antibody (25337-1-AP)

    WB analysis of HeLa using 25337-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RTEL1 Polyclonal antibody (25337-1-AP)

    WB analysis of mouse brain using 25337-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using RTEL1 Polyclonal antibody (25337-1-AP)

    WB analysis of PC-3 using 25337-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25337-1-AP (RTEL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using RTEL1 Polyclonal antibody (25337-1-AP)

    IP experiment of mouse brain using 25337-1-AP

    IP result of anti-RTEL1 (IP:25337-1-AP, 4ug; Detection:25337-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RTEL1 Polyclonal antibody (25337-1-AP)

    IF Staining of HepG2 using 25337-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 25337-1-AP (RTEL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inBGC-823 cells, C6 cells, HeLa cells, HepG2 cells, mouse brain tissue, mouse testis tissue, PC-3 cells
Positive IP detected inmouse brain tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25337-1-AP targets RTEL1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityzebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name regulator of telomere elongation helicase 1
Calculated Molecular Weight 142 kDa
Observed Molecular Weight 133 kDa
GenBank Accession NumberNM_016434
Gene Symbol RTEL1
Gene ID (NCBI) 51750
RRIDAB_2880034
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RTEL1 antibody 25337-1-APDownload protocol
IF protocol for RTEL1 antibody 25337-1-APDownload protocol
IP protocol for RTEL1 antibody 25337-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishWB

Nucleic Acids Res

Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing.

Authors - Jingzhen Li
View Article