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RTF1 Polyclonal antibody

RTF1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12170-1-AP

Synonyms

CDG1N, GTL7, KIAA0252, RTF1

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using RTF1 Polyclonal antibody (12170-1-AP)

    WB analysis of MCF-7 using 12170-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using RTF1 Polyclonal antibody (12170-1-AP)

    WB analysis of HL-60 using 12170-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using RTF1 Polyclonal antibody (12170-1-AP)

    WB analysis of rat brain using 12170-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using RTF1 Polyclonal antibody (12170-1-AP)

    WB analysis of mouse brain using 12170-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12170-1-AP (RTF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RTF1 Polyclonal antibody (12170-1-AP)

    IP experiment of HeLa using 12170-1-AP

    IP result of anti-RTF1 (IP:12170-1-AP, 4ug; Detection:12170-1-AP 1:500) with HeLa cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RTF1 Polyclonal antibody (12170-1-AP)

    IHC staining of human prostate cancer using 12170-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12170-1-AP (RTF1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RTF1 Polyclonal antibody (12170-1-AP)

    IHC staining of human prostate cancer using 12170-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12170-1-AP (RTF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inMCF-7 cells, HL-60 cells, rat brain tissue, mouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12170-1-AP targets RTF1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RTF1 fusion protein Ag2812
Full Name Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Calculated Molecular Weight585aa,67 kDa; 710aa,80 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC015052
Gene Symbol RTF1
Gene ID (NCBI) 23168
RRIDAB_2180931
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RTF1 antibody 12170-1-APDownload protocol
IHC protocol for RTF1 antibody 12170-1-APDownload protocol
IP protocol for RTF1 antibody 12170-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Cell cycle-resolved chromatin proteomics reveals the extent of mitotic preservation of the genomic regulatory landscape.

Authors - Paul Adrian Ginno
View Article
mouseWB

Cell Death Dis

UBR5 targets tumor suppressor CDC73 proteolytically to promote aggressive breast cancer.

Authors - Gang Xiang
View Article
mouseWB,IP

Cell

Inherited blood cancer predisposition through altered transcription elongation

Authors - Jiawei Zhao
View Article