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  • Featured Product
  • KD/KO Validated

SCIN Polyclonal antibody

SCIN Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11579-1-AP

Synonyms

Adseverin, KIAA1905, SCIN, scinderin

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using SCIN Polyclonal antibody (11579-1-AP)

    WB analysis of human placenta using 11579-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11579-1-AP (SCIN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human placenta tissue using SCIN Polyclonal antibody (11579-1-AP)

    IP experiment of human placenta using 11579-1-AP

    IP result of anti-SCIN (IP:11579-1-AP, 4ug; Detection:11579-1-AP 1:300) with human placenta tissue lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SCIN Polyclonal antibody (11579-1-AP)

    IHC staining of human pancreas cancer using 11579-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11579-1-AP (SCIN antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SCIN Polyclonal antibody (11579-1-AP)

    IF Staining of HepG2 using 11579-1-AP

    Immunofluorescent analysis of HepG2 cells, using SCIN antibody 11579-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11579-1-AP targets SCIN in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCIN fusion protein Ag2168
Full Name scinderin
Calculated Molecular Weight 715 aa, 81 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC021090
Gene Symbol SCIN
Gene ID (NCBI) 85477
RRIDAB_10641047
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SCIN antibody 11579-1-APDownload protocol
IHC protocol for SCIN antibody 11579-1-APDownload protocol
IF protocol for SCIN antibody 11579-1-APDownload protocol
IP protocol for SCIN antibody 11579-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

FEBS Open Bio

Loss of scinderin decreased expression of epidermal growth factor receptor and promoted apoptosis of castration-resistant prostate cancer cells.

Authors - Xiaofeng Lai
  • KD Validated
View Article
humanWB,IHC

World J Clin Oncol

Scinderin promotes glioma cell migration and invasion via remodeling actin cytoskeleton

Authors - Xin Lin
  • KD Validated
View Article
humanWB

Acta Neuropathol Commun

Synaptic proteomics reveal distinct molecular signatures of cognitive change and C9ORF72 repeat expansion in the human ALS cortex

Authors - Zsofia I. Laszlo
View Article