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SCN10A Polyclonal antibody

SCN10A Polyclonal Antibody for FC, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 55334-1-AP

Synonyms

hPN3, Nav1.8, PN3, SCN10A, SNS

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using SCN10A Polyclonal antibody (55334-1-AP)

    IF Staining of SH-SY5Y using 55334-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 55334-1-AP (SCN10A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using SCN10A Polyclonal antibody (55334-1-AP)

    FC experiment of HeLa using 55334-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SCN10A (55334-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inSH-SY5Y cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55334-1-AP targets SCN10A in IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name sodium channel, voltage-gated, type X, alpha subunit
Calculated Molecular Weight 221 kDa
GenBank Accession NumberNM_006514
Gene Symbol SCN10A
Gene ID (NCBI) 6336
RRIDAB_2881309
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for SCN10A antibody 55334-1-APDownload protocol
FC protocol for SCN10A antibody 55334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols