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SCUBE3 Polyclonal antibody

SCUBE3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16773-1-AP

Synonyms

CEGF3, SCUBE3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using SCUBE3 Polyclonal antibody (16773-1-AP)

    WB analysis of mouse kidney using 16773-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16773-1-AP (SCUBE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using SCUBE3 Polyclonal antibody (16773-1-AP)

    WB analysis of mouse lung using 16773-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 16773-1-AP (SCUBE3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using SCUBE3 Polyclonal antibody (16773-1-AP)

    IHC staining of human kidney using 16773-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16773-1-AP (SCUBE3 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SCUBE3 Polyclonal antibody (16773-1-AP)

    IHC staining of human kidney using 16773-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16773-1-AP (SCUBE3 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse kidney tissue, mouse lung tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16773-1-AP targets SCUBE3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCUBE3 fusion protein Ag10085
Full Name signal peptide, CUB domain, EGF-like 3
Calculated Molecular Weight 992 aa, 109 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC052263
Gene Symbol SCUBE3
Gene ID (NCBI) 222663
RRIDAB_2285430
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCUBE3 is a secreted glycoprotein that belongs to the SCUBE family. Members of the SCUBE family are secreted plasma membrane-associated proteins characterised by a N-terminal signal peptide sequence, multiple EGF domains, a N-linked glycosylated spacer region and a C-terminal CUB region. SCUBE3 may play a role in bone cell biology (PMID: 15234972). It may also has a role in the regulation of cardiac growth and remodeling responses (PMID: 17442284). Besides, SCUBE3 has been reported to be involved in lung cancer progression (PMID: 21441952; 23420440).

Protocols

Product Specific Protocols
WB protocol for SCUBE3 antibody 16773-1-APDownload protocol
IHC protocol for SCUBE3 antibody 16773-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Gene

Comprehensive and in-depth analysis of microRNA and mRNA expression profile in salivary adenoid cystic carcinoma.

Authors - Nannan Han
View Article
human,mouseWB

Mol Cell Endocrinol

β-catenin/TCF4-induced SCUBE3 upregulation promotes ovarian cancer development via HIF-1 signaling pathway

Authors - Jing Shen
  • KD Validated
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Boyan (Verified Customer) (10-04-2021)

Calculated band is 109 kd. This antibody recognized a major band at 60 kd. There was a very weak band ~130 kd, but was PNGaseF insensitive, suggesting this band is not a glycosylated form of this protein.

  • Applications: Western Blot