SDC2 Monoclonal antibody

SDC2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A7B2

Cat no : 67088-1-Ig

Synonyms

Fibroglycan, HSPG, HSPG1, SDC2, SYND2, syndecan 2



Tested Applications

Positive WB detected inL02 cells, HuH-7 cells, HepG2 cells, A549 cells, NCI-H1299 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67088-1-Ig targets SDC2 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SDC2 fusion protein Ag5739
Full Name syndecan 2
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 19 kDa, 22 and 48 kDa
GenBank Accession NumberBC049836
Gene Symbol SDC2
Gene ID (NCBI) 6383
RRIDAB_2882395
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SDC2, containing cell surface heparan sulphate proteoglycan, functions as an integral membrane protein and participates in cell proliferation, cell migration, cell adhesion and signal transduction. It has been reported that the expression level of SDC2 will be up-regulated under hypoxia condition which can induce increased release of cytokines and chemokines. Besides, methylated SDC2 can be used as a potential biomarker for early diagnosis of colon cancer. 67088-1-Ig antibody detects the 19 and 22 kDa SDC2 isoforms as well as the 48 kDa dimeric protein in SDS-PAGE.(PMID: 18803305, 23297089, 30022330, 29945573)

Protocols

Product Specific Protocols
WB protocol for SDC2 antibody 67088-1-IgDownload protocol
IHC protocol for SDC2 antibody 67088-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigIF

Sci Bull (Beijing)

Single-cell dynamics of liver development in postnatal pigs

Authors - Lin Rao
humanWB

Cell Oncol (Dordr)

Circular RNA circNFATC3 acts as a miR-9-5p sponge to promote cervical cancer development by upregulating SDC2.

Authors - Ningye Ma
humanWB

J Cell Mol Med

miR-218 affects the ECM composition and cell biomechanical properties of glioblastoma cells.

Authors - Małgorzata Grabowska
humanIHC

Cancer Cell Int

Identification of an immune-related signature indicating the dedifferentiation of thyroid cells.

Authors - Xuemin Wang
humanWB

Cancer Cell Int

GNG5 is a novel oncogene associated with cell migration, proliferation, and poor prognosis in glioma.

Authors - Wang Zhang
humanWB

Heart Vessels

Downregulation of lncRNA SNHG16 inhibits vascular smooth muscle cell proliferation and migration in cerebral atherosclerosis by targeting the miR-30c-5p/SDC2 axis.

Authors - Sheng-Nan Yang