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SEMA7A Polyclonal antibody

SEMA7A Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 18070-1-AP

Synonyms

CD108, CDw108, H SEMA K1, H Sema L, JMH, JMH blood group antigen, Sema K1, Sema L, SEMA7A, SEMAK1, SEMAL, Semaphorin 7A, Semaphorin K1, Semaphorin L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEMA7A Polyclonal antibody (18070-1-AP)

    WB analysis using 18070-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18070-1-AP (SEMA7A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human placenta tissue using SEMA7A Polyclonal antibody (18070-1-AP)

    IP experiment of human placenta using 18070-1-AP

    IP result of anti-SEMA7A (IP:18070-1-AP, 4ug; Detection:18070-1-AP 1:500) with human placenta tissue lysate 2600 ug.

Tested Applications

Positive WB detected inhuman placenta tissue, mouse brain tissue
Positive IP detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

18070-1-AP targets SEMA7A in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEMA7A fusion protein Ag12707
Full Name semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group)
Calculated Molecular Weight 666 aa, 75 kDa
Observed Molecular Weight75-80 kDa
GenBank Accession NumberBC101643
Gene Symbol SEMA7A
Gene ID (NCBI) 8482
RRIDAB_2878489
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEMA7A, also named CD108 and SEMAL, belongs to the semaphorin family. SEMA7A is the only membrane‐associated glycosylphosphatidylinositol (GPI)‐linked semaphorin and plays a role in the regulation of neuronal axon outgrowth. In addition to its role in guiding axon pathfinding during neuronal development, Sema7A has diverse functions in morphogenesis and immune cell control and regulates T cell responses via the α1β1 integrin receptor (PMID: 31179640). SEMA7A has 2 isoforms with molecular weights of 73 and 75 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for SEMA7A antibody 18070-1-APDownload protocol
IP protocol for SEMA7A antibody 18070-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Clin Invest

EMT-activated secretory and endocytic vesicular trafficking programs underlie a vulnerability to PI4K2A antagonism in lung cancer

Authors - Xiaochao Tan
View Article
mouseWB

Mol Metab

Sema7A protects against high-fat diet-induced obesity and hepatic steatosis by regulating adipo/lipogenesis

Authors - Qiongyu Lu
View Article