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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Septin 2 Monoclonal antibody

Septin 2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F3B2

Cat no : CL594-60075

Synonyms

DIFF6, hNedd5, KIAA0158, NEDD 5, NEDD5, Pnutl3, SEPT2, Septin 2, septin2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of K-562 cells using CoraLite®594-conjugated Septin 2 Monoclonal antibo (CL594-60075)

    FC experiment of K-562 using CL594-60075

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Septin 2 (CL594-60075, Clone:2F3B2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60075 targets Septin 2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Septin 2 fusion protein Ag1946
Full Name septin 2
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 42-45 kDa
GenBank Accession NumberBC014455
Gene Symbol SEPT2
Gene ID (NCBI) 4735
RRIDAB_2883416
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Septin 2, also named as DIFF6, KIAA0158, NEDD5, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. Septin 2 is coordinated expression with Septin 6 and Septin 7. (PMID:21737677)

Protocols

Product Specific Protocols
FC protocol for CL594 Septin 2 antibody CL594-60075Download protocol
Standard Protocols
Click here to view our Standard Protocols