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Alpha Sarcoglycan Polyclonal antibody

Alpha sarcoglycan Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 13222-1-AP

Synonyms

50 DAG, 50DAG, A2, adhalin, ADL, Alpha sarcoglycan, Alpha SG, DAG2, DMDA2, Dystroglycan 2, LGMD2D, SCARMD1, SGCA

Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using Alpha Sarcoglycan Polyclonal antibody (13222-1-AP)

    WB analysis of human skeletal muscle using 13222-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13222-1-AP (Alpha-sarcoglycan antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13222-1-AP targets Alpha sarcoglycan in WB,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha sarcoglycan fusion protein Ag3924
Full Name sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein)
Calculated Molecular Weight 387 aa, 43 kDa
GenBank Accession NumberBC025702
Gene Symbol SGCA
Gene ID (NCBI) 6442
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha-sarcoglycan is a member of the sarcoglycan-sarcospan complex (SG-SSPN), which is constituted by α/ɛ-, β-, γ-, and δ-SGs as well as SSPN. Alpha-sarcoglycan gene expression is exclusive of striated muscle and is finely regulated during myogenic differentiation, where the amount of alpha-sarcoglycan transcript is selectively increased after differentiation to myotubes (MTs). Alpha sarcoglycan's disruption causes limb-girdle muscular dystrophy.

Protocols

Product Specific Protocols
WB protocol for Alpha sarcoglycan antibody 13222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols