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SHC3 Polyclonal antibody

SHC3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12436-1-AP

Synonyms

N Shc, Neuronal Shc, NSHC, Protein Rai, RAI, SH2 domain protein C3, SHC transforming protein 3, SHC transforming protein C, SHC3, SHCC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SHC3 Polyclonal antibody (12436-1-AP)

    WB analysis using 12436-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12436-1-AP (SHC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of BxPC-3 cells using SHC3 Polyclonal antibody (12436-1-AP)

    IP experiment of BxPC-3 using 12436-1-AP

    IP result of anti-SHC3 (IP:12436-1-AP, 4ug; Detection:12436-1-AP 1:300) with BxPC-3 cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SHC3 Polyclonal antibody (12436-1-AP)

    IHC staining of mouse pancreas using 12436-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12436-1-AP (SHC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using SHC3 Polyclonal antibody (12436-1-AP)

    IHC staining of mouse pancreas using 12436-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12436-1-AP (SHC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inBxPC-3 cells
Positive IP detected inBxPC-3 cells
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12436-1-AP targets SHC3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHC3 fusion protein Ag3115
Full Name SHC (Src homology 2 domain containing) transforming protein 3
Calculated Molecular Weight 594 aa, 64 kDa
Observed Molecular Weight 50-52 kDa
GenBank Accession NumberBC026314
Gene Symbol SHC3
Gene ID (NCBI) 53358
RRIDAB_2301798
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SHC3 antibody 12436-1-APDownload protocol
IHC protocol for SHC3 antibody 12436-1-APDownload protocol
IP protocol for SHC3 antibody 12436-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Lysate microarrays enable high-throughput, quantitative investigations of cellular signaling.

Authors - Mark Sevecka
View Article
mouseIHC

J Hepatocell Carcinoma

The Long Non-Coding RNA AC006329.1 Facilitates Hepatocellular Carcinoma Progression and Metastasis by Regulating miR-127-5p/SHC3/ERK Axis

Authors - Xiang Xu Kong
View Article