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SIM2 Polyclonal antibody

SIM2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 21069-1-AP

Synonyms

bHLHe15, SIM, SIM2, Single minded homolog 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SIM2 Polyclonal antibody (21069-1-AP)

    WB analysis using 21069-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21069-1-AP (SIM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using SIM2 Polyclonal antibody (21069-1-AP)

    WB analysis of human skeletal muscle using 21069-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21069-1-AP (SIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using SIM2 Polyclonal antibody (21069-1-AP)

    IP experiment of mouse skeletal muscle using 21069-1-AP

    IP result of anti-SIM2 (IP:21069-1-AP, 4ug; Detection:21069-1-AP 1:800) with mouse skeletal muscle tissue lysate 2200ug.

Tested Applications

Positive WB detected inCOLO 320 cells, human skeletal muscle tissue, HeLa cells, HepG2 cells, U-251 cells
Positive IP detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

ChIPSee 1 publications below

Product Information

21069-1-AP targets SIM2 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIM2 fusion protein Ag15204
Full Name single-minded homolog 2 (Drosophila)
Calculated Molecular Weight 667 aa, 73 kDa
Observed Molecular Weight 60-64 kDa
GenBank Accession NumberBC110444
Gene Symbol SIM2
Gene ID (NCBI) 6493
RRIDAB_10732844
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIM2, also named as single-minded homolog 2, is a 667 amino acid protein, which contains 1 bHLH (basic helix-loop-helix) domain and one PAS-associated C-terminal domain. SIM2 localizes in the nucleus and has tow isoforms. Isoform SIM2 is a 73 kDa protein and Isoform SIM2S is 63 kDa protein. SIM2 as a transcription factor may be a master gene of CNS development in cooperation with Arnt. It may have pleiotropic effects in the tissues expressed during development.

Protocols

Product Specific Protocols
WB protocol for SIM2 antibody 21069-1-APDownload protocol
IP protocol for SIM2 antibody 21069-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanChIP

Biochem J

Characterization of functionally deficient SIM2 variants found in patients with neurological phenotypes.

Authors - Emily Lyn Button
View Article