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SLC28A3 Polyclonal antibody

SLC28A3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18182-1-AP

Synonyms

CNT 3, CNT3, hCNT3, SLC28A3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC28A3 Polyclonal antibody (18182-1-AP)

    WB analysis using 18182-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18182-1-AP (SLC28A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SLC28A3 Polyclonal antibody (18182-1-AP)

    IHC staining of human pancreas cancer using 18182-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18182-1-AP (SLC28A3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SLC28A3 Polyclonal antibody (18182-1-AP)

    IHC staining of human pancreas cancer using 18182-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18182-1-AP (SLC28A3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SLC28A3 Polyclonal antibody (18182-1-AP)

    IF Staining of HepG2 using 18182-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SLC28A3 antibody (18182-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHL-60 cells, mouse kidney tissue, mouse liver tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18182-1-AP targets SLC28A3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC28A3 fusion protein Ag12887
Full Name solute carrier family 28 (sodium-coupled nucleoside transporter), member 3
Calculated Molecular Weight 691 aa, 77 kDa
Observed Molecular Weight70-75 kDa
GenBank Accession NumberBC093821
Gene Symbol SLC28A3
Gene ID (NCBI) 64078
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC28A3 (Solute carrier family 28 member 3), also named CNT3, has sodium-dependent, pyrimidine- and purine-selective (PMID: 11032837, PMID: 15861042, PMID: 16446384, PMID: 17140564, PMID: 21998139). It is involved in the homeostasis of endogenous nucleosides (PMID: 11032837, PMID: 15861042). SLC28A3 is able to transports uridine, gemcitabine, 3'-azido-3'-deoxythymidine (AZT), ribavirin and 3-deazauridine (PMID: 21795683, PMID: 11032837, PMID: 17140564). SLC28A3, the human concentrative nucleoside transporter, plays a role in mediating the cellular entry of a broad array of physiological nucleosides and synthetic anticancer nucleoside analog drugs (PMID: 15738947). Variants in SLC28A3 and UGT1A6 act as genetic markers predictive of anthracycline-induced cardiotoxicity (PMID: 23441093).

Protocols

Product Specific Protocols
WB protocol for SLC28A3 antibody 18182-1-APDownload protocol
IHC protocol for SLC28A3 antibody 18182-1-APDownload protocol
IF protocol for SLC28A3 antibody 18182-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols