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SLC31A1 Polyclonal antibody

SLC31A1 Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 27499-1-AP

Synonyms

Copper transporter 1, COPT1, CTR1, hCTR1, SLC31A1

Validation Data Gallery

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  • Western Blot (WB) analysis of HT-29 cells using SLC31A1 Polyclonal antibody (27499-1-AP)

    WB analysis of HT-29 using 27499-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 27499-1-AP (SLC31A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SLC31A1 Polyclonal antibody (27499-1-AP)

    WB analysis of HeLa using 27499-1-AP

    WB result of SLC31A1 antibody (27499-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC31A1 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using SLC31A1 Polyclonal antibody (27499-1-AP)

    WB analysis using 27499-1-AP

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 27499-1-AP (SLC31A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using SLC31A1 Polyclonal antibody (27499-1-AP)

    IF Staining of human liver cancer using 27499-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SLC31A1 antibody (27499-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using SLC31A1 Polyclonal antibody (27499-1-AP)

    FC experiment of HeLa using 27499-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SLC31A1 (27499-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of Neuro-2a cells using SLC31A1 Polyclonal antibody (27499-1-AP)

    FC experiment of Neuro-2a using 27499-1-AP

    1X10^6 Neuro-2a cells were intracellularly stained with 0.4 ug Anti-Human SLC31A1 (27499-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using SLC31A1 Polyclonal antibody (27499-1-AP)

    FC experiment of HeLa using 27499-1-AP

    1X10^6 HeLa cells were surface stained with 0.4 ug Anti-Human SLC31A1 (27499-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were not fixed .

Tested Applications

Positive WB detected inHT-29 cells, Caco-2 cells, HeLa cells
Positive IF detected inhuman liver cancer tissue
Positive FC detected inHeLa cells, Neuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

27499-1-AP targets SLC31A1 in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC31A1 fusion protein Ag26601
Full Name solute carrier family 31 (copper transporters), member 1
Calculated Molecular Weight 21 kDa
Observed Molecular Weight21-25,30-35,60-70 kDa
GenBank Accession NumberBC013611
Gene Symbol SLC31A1
Gene ID (NCBI) 1317
RRIDAB_2918124
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SLC31A1 antibody 27499-1-APDownload protocol
IF protocol for SLC31A1 antibody 27499-1-APDownload protocol
FC protocol for SLC31A1 antibody 27499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Int J Mol Sci

Transcriptional Responses of Copper-Transport-Related Genes ctr1, ctr2 and atox1 and Their Roles in the Regulation of Cu Homeostasis in Yellow Catfish Pelteobagrus fulvidraco

Authors - Hong Yang
View Article
humanWB

Cancer Res Commun

Targeting NEDDylation is a Novel Strategy to Attenuate Cisplatin-induced Nephrotoxicity

Authors - Trace M Jones
View Article