SLC40A1/FPN1 Polyclonal antibody
SLC40A1/FPN1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse and More (2)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 26601-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Caco-2 cells, mouse spleen tissue, HepG2 cells, mouse liver tissue, rat spleen, HEK-293T cells, SW480 cells |
| Positive IHC detected in | human liver tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 52 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
Product Information
26601-1-AP targets SLC40A1/FPN1 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human, mouse, rat, chicken, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SLC40A1/FPN1 fusion protein Ag24273 |
| Full Name | solute carrier family 40 (iron-regulated transporter), member 1 |
| Calculated Molecular Weight | 62 kDa |
| Observed Molecular Weight | 62-70 kDa |
| GenBank Accession Number | BC037733 |
| Gene Symbol | SLC40A1 |
| Gene ID (NCBI) | 30061 |
| RRID | AB_2880571 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SLC40A1, also named as Ferroportin 1 (FPN1), is an iron transporter which is involved in iron export from duodenal epithelial cell and also in transfer of iron between maternal and fetal circulation. It has been reported that SLC40A1 mutations may lead to macrophage iron overload, hyperferritinemia, and normal transferrin saturation among ferroportin iron overload patients. This antibody detects 62-70 kDa protein as well as the fragments of 56 kDa and 35 kDa protein similar to papers in SDS-PAGE. (PMID: 21396368,16054062, 18974313)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SLC40A1/FPN1 antibody 26601-1-AP | Download protocol |
| IHC protocol for SLC40A1/FPN1 antibody 26601-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Redox Biol NCOA4-mediated ferritinophagy is involved in ionizing radiation-induced ferroptosis of intestinal epithelial cells. | ||
Cell Death Dis Super-enhancer-driven MLX mediates redox balance maintenance via SLC7A11 in osteosarcoma | ||
Theranostics Holo-lactoferrin: the link between ferroptosis and radiotherapy in triple-negative breast cancer. | ||
Redox Biol miR-30-5p-mediated ferroptosis of trophoblasts is implicated in the pathogenesis of preeclampsia. | ||
Cell Chem Biol 3D two-photon brain imaging reveals dihydroartemisinin exerts antiepileptic effects by modulating iron homeostasis. | ||
Oxid Med Cell Longev HO-1 Contributes to Luteolin-Triggered Ferroptosis in Clear Cell Renal Cell Carcinoma via Increasing the Labile Iron Pool and Promoting Lipid Peroxidation. |
