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  • Featured Product
  • KD/KO Validated

NHE8 Polyclonal antibody

NHE8 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18318-1-AP

Synonyms

KIAA0939, Na(+)/H(+) exchanger 8, NHE 8, NHE8, SLC9A8, Sodium/hydrogen exchanger 8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NHE8 Polyclonal antibody (18318-1-AP)

    WB analysis using 18318-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18318-1-AP (NHE8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NHE8 Polyclonal antibody (18318-1-AP)

    WB analysis of HEK-293 using 18318-1-AP

    WB result of NHE8 antibody (18318-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NHE8 transfected HEK-293 cells.

  • Western Blot (WB) analysis of mouse liver tissue using NHE8 Polyclonal antibody (18318-1-AP)

    WB analysis of mouse liver using 18318-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 18318-1-AP (NHE8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using NHE8 Polyclonal antibody (18318-1-AP)

    WB analysis of mouse kidney using 18318-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18318-1-AP (NHE8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using NHE8 Polyclonal antibody (18318-1-AP)

    WB analysis of mouse testis using 18318-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 18318-1-AP (NHE8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NHE8 Polyclonal antibody (18318-1-AP)

    IHC staining of human kidney using 18318-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NHE8 Polyclonal antibody (18318-1-AP)

    IHC staining of human kidney using 18318-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NHE8 Polyclonal antibody (18318-1-AP)

    IHC staining of mouse testis using 18318-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using NHE8 Polyclonal antibody (18318-1-AP)

    IHC staining of mouse testis using 18318-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NHE8 Polyclonal antibody (18318-1-AP)

    IHC staining of human kidney using 18318-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NHE8 Polyclonal antibody (18318-1-AP)

    IHC staining of human kidney using 18318-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using NHE8 Polyclonal antibody (18318-1-AP)

    IHC staining of human colon using 18318-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using NHE8 Polyclonal antibody (18318-1-AP)

    IHC staining of human colon using 18318-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18318-1-AP (NHE8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using NHE8 Polyclonal antibody (18318-1-AP)

    IHC staining of human placenta using 18318-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using NHE8 Polyclonal antibody (18318-1-AP)

    IHC staining of human placenta using 18318-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using NHE8 Polyclonal antibody (18318-1-AP)

    IHC staining of human testis using 18318-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using NHE8 Polyclonal antibody (18318-1-AP)

    IHC staining of human testis using 18318-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using NHE8 Polyclonal antibody (18318-1-AP)

    IHC staining of human skin using 18318-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using NHE8 Polyclonal antibody (18318-1-AP)

    IHC staining of human skin using 18318-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using NHE8 Polyclonal antibody (18318-1-AP)

    IHC staining of human lung using 18318-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using NHE8 Polyclonal antibody (18318-1-AP)

    IHC staining of human lung using 18318-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using NHE8 Polyclonal antibody (18318-1-AP)

    IHC staining of human ovary using 18318-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18318-1-AP (NHE8 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using NHE8 Polyclonal antibody (18318-1-AP)

    IF Staining of HEK-293 using 18318-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 18318-1-AP (NHE8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, mouse testis tissue, mouse colon tissue, mouse liver tissue, mouse kidney tissue
Positive IHC detected inhuman kidney tissue, human colon tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18318-1-AP targets NHE8 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NHE8 fusion protein Ag13111
Full Name solute carrier family 9 (sodium/hydrogen exchanger), member 8
Calculated Molecular Weight 581 aa, 65 kDa
Observed Molecular Weight 70-85 kDa
GenBank Accession NumberBC112213
Gene Symbol SLC9A8
Gene ID (NCBI) 23315
RRIDAB_2270442
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NHE8, encoded by SLC9A8, is a member of sodium/hydrogen exchanger family which are transmembrane proteins that mediate the electroneutral exchange of sodium (Na) for hydrogen (H) and get involved in intracellular pH homeostasis, cell volume regulation, acid-base regulation, and so on. NHE8 has a broad tissue distribution, with relatively high abundance in the gastrointestinal tract. It has been reported that NHE8 expression differs among different regions in the stomach, very low in nonglandular region whereas very high in glandular region. The predicted molecular weight of NHE8 is 64-65 kDa, while its glycosylated form often migrates around 70-85 kDa.

Protocols

Product Specific Protocols
WB protocol for NHE8 antibody 18318-1-APDownload protocol
IHC protocol for NHE8 antibody 18318-1-APDownload protocol
IF protocol for NHE8 antibody 18318-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols