SMO Monoclonal antibody
SMO Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Canine, Mouse, Rat and More (3)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
2B10A11
Cat no : 66851-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 19 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
66851-1-Ig targets SMO in WB, IHC, IF, ELISA applications and shows reactivity with Human, Canine, Mouse, Rat samples.
| Tested Reactivity | Human, Canine, Mouse, Rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | SMO fusion protein Ag25861 |
| Full Name | smoothened homolog (Drosophila) |
| Calculated Molecular Weight | 787 aa, 86 kDa |
| Observed Molecular Weight | 86 kDa |
| GenBank Accession Number | BC009989 |
| Gene Symbol | SMO |
| Gene ID (NCBI) | 6608 |
| RRID | AB_2882191 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays central roles in animal development and stem-cell function. Defects in Hh signalling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SMO antibody 66851-1-Ig | Download protocol |
| IHC protocol for SMO antibody 66851-1-Ig | Download protocol |
| IF protocol for SMO antibody 66851-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Drug Repurposing Flubendazole to Suppress Tumorigenicity via PCSK9-dependent Inhibition and Potentiate Lenvatinib Therapy for Hepatocellular Carcinoma | ||
iScience Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1 | ||
Front Cell Dev Biol Microtubule Stabilization Enhances the Chondrogenesis of Synovial Mesenchymal Stem Cells. | ||
Oxid Med Cell Longev Mitochondrial Protection and Against Glutamate Neurotoxicity via Shh/Ptch1 Signaling Pathway to Ameliorate Cognitive Dysfunction by Kaixin San in Multi-Infarct Dementia Rats | ||
Cancer Sci Transcription factor POU4F2 promotes colorectal cancer cell migration and invasion through hedgehog-mediated epithelial-mesenchymal transition. | ||
FASEB J Inhibition of CK2α accelerates skin wound healing by promoting endothelial cell proliferation through the Hedgehog signaling pathway |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Aidan (Verified Customer) (08-31-2021) | Smo Monoclonal antibody responded to S. purpuratus Smo protein in both IF and WB experiments. For IF, methanol fixed embryos treated with 1:100 dilution of the antibody in PBS overnight, followed by incubation in a 1:300 dilution of secondary antibody Alexa Goat anti-mouse 488 diluted 1:300 in PBS. Localization appears peri-nuclear at the 16 cell stage.
 |
FH Boyan (Verified Customer) (04-24-2019) | By WB, this antibody primarily labeled a band around 110 kD; by IF, it cannot label the ciliary localisation of Smo when the cells were stimulated for Hh pathway.
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