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SMTNL2 Polyclonal antibody

SMTNL2 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 14782-1-AP

Synonyms

smoothelin like 2, Smoothelin like protein 2, SMTNL2

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using SMTNL2 Polyclonal antibody (14782-1-AP)

    WB analysis of COLO 320 using 14782-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using SMTNL2 Polyclonal antibody (14782-1-AP)

    WB analysis of mouse small intestine using 14782-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using SMTNL2 Polyclonal antibody (14782-1-AP)

    WB analysis of MCF-7 using 14782-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SGC-7901 cells using SMTNL2 Polyclonal antibody (14782-1-AP)

    WB analysis of SGC-7901 using 14782-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using SMTNL2 Polyclonal antibody (14782-1-AP)

    IP experiment of COLO 320 using 14782-1-AP

    IP result of anti-SMTNL2 (IP:14782-1-AP, 4ug; Detection:14782-1-AP 1:500) with COLO 320 cells lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SMTNL2 Polyclonal antibody (14782-1-AP)

    IF Staining of MCF-7 using 14782-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 14782-1-AP (SMTNL2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inCOLO 320 cells, MCF-7 cells, mouse small intestine tissue, SGC-7901 cells
Positive IP detected inCOLO 320 cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14782-1-AP targets SMTNL2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMTNL2 fusion protein Ag6395
Full Name smoothelin-like 2
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 68-70 kDa
GenBank Accession NumberBC112177
Gene Symbol SMTNL2
Gene ID (NCBI) 342527
RRIDAB_2270842
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SMTNL2 antibody 14782-1-APDownload protocol
IF protocol for SMTNL2 antibody 14782-1-APDownload protocol
IP protocol for SMTNL2 antibody 14782-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols