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SPACA1 Polyclonal antibody

SPACA1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12829-1-AP

Synonyms

SAMP32, SPACA1, sperm acrosome associated 1

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human testis tissue using SPACA1 Polyclonal antibody (12829-1-AP)

    IHC staining of human testis using 12829-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12829-1-AP (SPACA1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using SPACA1 Polyclonal antibody (12829-1-AP)

    IHC staining of human testis using 12829-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12829-1-AP (SPACA1 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12829-1-AP targets SPACA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPACA1 fusion protein Ag3549
Full Name sperm acrosome associated 1
Calculated Molecular Weight 294 aa, 32 kDa
GenBank Accession NumberBC029488
Gene Symbol SPACA1
Gene ID (NCBI) 81833
RRIDAB_2877886
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPACA1, also named as SAMP32, is a membrane protein that localizes in the equatorial segment of spermatozoa in mammals and is reported to function in sperm-egg fusion. SPACA1 is regulated by phosphorylation.

Protocols

Product Specific Protocols
IHC protocol for SPACA1 antibody 12829-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Free Radic Res

Heterozygous SOD1 deficiency in mice with an NZW background causes male infertility and an aberrant immune phenotype

Authors - Takujiro Homma
View Article