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SPARCL1 Monoclonal antibody

SPARCL1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A9E7

Cat no : 67511-1-Ig

Synonyms

Hevin, MAST 9, PIG33, SC1, SPARC like 1 (hevin), SPARC like protein 1, SPARCL1

Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using SPARCL1 Monoclonal antibody (67511-1-Ig)

    WB analysis of human saliva using 67511-1-Ig

    human saliva were subjected to SDS PAGE followed by western blot with 67511-1-Ig (SPARCL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using SPARCL1 Monoclonal antibody (67511-1-Ig)

    WB analysis of Raji using 67511-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 67511-1-Ig (SPARCL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman saliva, Raji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67511-1-Ig targets SPARCL1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SPARCL1 fusion protein Ag29092
Full Name SPARC-like 1 (hevin)
Calculated Molecular Weight 664 aa, 75 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC033721
Gene Symbol SPARCL1
Gene ID (NCBI) 8404
RRIDAB_2882732
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPARC-like protein 1 (SPARCL1), also known as SC1 or Hevin, is an extracellular matrix glycoprotein that belongs to the SPARC family. SPARCL1 is widely expressed in normal and cancer tissues, and has been shown to regulate cellular adhesion and migration. SPARCL1 has been implicated in tumor initiation, formation, and progression of various cancers, including pancreatic, prostate, bladder, ovarian, breast and non-small cell lung cancers.

Protocols

Product Specific Protocols
WB protocol for SPARCL1 antibody 67511-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols