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SPATA16 Polyclonal antibody

SPATA16 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 17357-1-AP

Synonyms

NYD SP12, SPATA16, spermatogenesis associated 16

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human testis tissue using SPATA16 Polyclonal antibody (17357-1-AP)

    IHC staining of human testis using 17357-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17357-1-AP (SPATA16 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using SPATA16 Polyclonal antibody (17357-1-AP)

    IHC staining of human testis using 17357-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17357-1-AP (SPATA16 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17357-1-AP targets SPATA16 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPATA16 fusion protein Ag10637
Full Name spermatogenesis associated 16
Calculated Molecular Weight 569 aa, 65 kDa
GenBank Accession NumberBC034496
Gene Symbol SPATA16
Gene ID (NCBI) 83893
RRIDAB_2878396
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPATA16, also named as NYD-SP12, belongs to the SPATA16 family. It is involved in the formation of sperm acrosome, which implicated its potential role in spermatogenesis and sperm-egg fusion. SPATA16 localizes to Golgi apparatus and is primarily expressed in testis, with lower levels found in kidney and pancreas.

Protocols

Product Specific Protocols
IHC protocol for SPATA16 antibody 17357-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols