• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

SPIN1-Specific Polyclonal antibody

SPIN1-Specific Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19531-1-AP

Synonyms

OCR, Ovarian cancer related protein, SPIN, SPIN1, SPIN1-Specific, spindlin 1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of SKOV-3 cells using SPIN1-Specific Polyclonal antibody (19531-1-AP)

    WB analysis of SKOV-3 using 19531-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 19531-1-AP (SPIN1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SPIN1-Specific Polyclonal antibody (19531-1-AP)

    WB analysis of HEK-293 using 19531-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19531-1-AP (SPIN1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using SPIN1-Specific Polyclonal antibody (19531-1-AP)

    WB analysis of A2780 using 19531-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 19531-1-AP (SPIN1-Specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using SPIN1-Specific Polyclonal antibody (19531-1-AP)

    IHC staining of human ovary tumor using 19531-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 19531-1-AP (SPIN1-Specific antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using SPIN1-Specific Polyclonal antibody (19531-1-AP)

    IHC staining of human ovary tumor using 19531-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 19531-1-AP (SPIN1-Specific antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SPIN1-Specific Polyclonal antibody (19531-1-AP)

    IF Staining of HeLa using 19531-1-AP

    Immunofluorescent analysis of Hela cells, using SPIN1 antibody 19531-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inSKOV-3 cells, A2780 cells, HEK-293 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19531-1-AP targets SPIN1-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name spindlin 1
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberNM_006717
Gene Symbol SPIN1
Gene ID (NCBI) 10927
RRIDAB_10638616
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPIN1 (Spindlin 1) is a histone methylation reader, which recognizes trimethylated histone H3 lysine 4 through the protein-protein interaction. SPIN1 was initially found in ovarian cancer via searching ovarian cancer-related genes . It is overexpressed in ovarian cancer tissue, which exhibits a high homology to mouse Spin (Spindlin) gene. Signaling pathways responsible for SPIN1 functions include PI3K/Akt, Wnt and RET that are highly relevant to tumorigenesis.(PMID: 34492335, PMID: 31790762)

Protocols

Product Specific Protocols
WB protocol for SPIN1-Specific antibody 19531-1-APDownload protocol
IHC protocol for SPIN1-Specific antibody 19531-1-APDownload protocol
IF protocol for SPIN1-Specific antibody 19531-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Suppressive role exerted by microRNA-29b-1-5p in triple negative breast cancer through SPIN1 regulation.

Authors - Rosa Drago-Ferrante
  • KD Validated
View Article
humanWB

J Cell Biochem

Molecular basis underlying the biological effects elicited by extremely low-frequency magnetic field (ELF-MF) on neuroblastoma cells.

Authors - Sulpizio Marilisa M
View Article
humanIHC

Bioengineered

The microRNA-381(miR-381)/Spindlin1(SPIN1) axis contributes to cell proliferation and invasion of colorectal cancer cells by regulating the Wnt/β-catenin pathway.

Authors - Ling Zhou
View Article
humanIHC

J Exp Clin Cancer Res

SPIN1, negatively regulated by miR-148/152, enhances Adriamycin resistance via upregulating drug metabolizing enzymes and transporter in breast cancer.

Authors - Xu Chen
View Article