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SPZ1 Polyclonal antibody

SPZ1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18931-1-AP

Synonyms

NYD TSP1, spermatogenic leucine zipper 1, SPZ1, Testis specific protein 1, TSP1

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using SPZ1 Polyclonal antibody (18931-1-AP)

    WB analysis of PC-3 using 18931-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 18931-1-AP (SPZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DU 145 cells using SPZ1 Polyclonal antibody (18931-1-AP)

    WB analysis of DU 145 using 18931-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 18931-1-AP (SPZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using SPZ1 Polyclonal antibody (18931-1-AP)

    WB analysis of human testis using 18931-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 18931-1-AP (SPZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using SPZ1 Polyclonal antibody (18931-1-AP)

    IHC staining of human testis using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using SPZ1 Polyclonal antibody (18931-1-AP)

    IHC staining of human testis using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using SPZ1 Polyclonal antibody (18931-1-AP)

    IHC staining of human skin using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using SPZ1 Polyclonal antibody (18931-1-AP)

    IHC staining of human skin using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using SPZ1 Polyclonal antibody (18931-1-AP)

    IHC staining of human brain using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using SPZ1 Polyclonal antibody (18931-1-AP)

    IHC staining of human brain using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using SPZ1 Polyclonal antibody (18931-1-AP)

    IHC staining of human spleen using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using SPZ1 Polyclonal antibody (18931-1-AP)

    IHC staining of human spleen using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using SPZ1 Polyclonal antibody (18931-1-AP)

    IHC staining of human lung using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using SPZ1 Polyclonal antibody (18931-1-AP)

    IHC staining of human lung using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using SPZ1 Polyclonal antibody (18931-1-AP)

    IHC staining of human ovary using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using SPZ1 Polyclonal antibody (18931-1-AP)

    IHC staining of human ovary using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using SPZ1 Polyclonal antibody (18931-1-AP)

    IHC staining of human brain using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using SPZ1 Polyclonal antibody (18931-1-AP)

    IHC staining of human brain using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SPZ1 Polyclonal antibody (18931-1-AP)

    IHC staining of human kidney using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SPZ1 Polyclonal antibody (18931-1-AP)

    IHC staining of human kidney using 18931-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18931-1-AP (SPZ1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using SPZ1 Polyclonal antibody (18931-1-AP)

    IF Staining of PC-3 using 18931-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 18931-1-AP (SPZ1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inPC-3 cells, DU 145 cells, human testis tissue
Positive IHC detected inhuman testis tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18931-1-AP targets SPZ1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPZ1 fusion protein Ag6995
Full Name spermatogenic leucine zipper 1
Calculated Molecular Weight 430 aa, 49 kDa
Observed Molecular Weight 49-55 kDa
GenBank Accession NumberBC033798
Gene Symbol SPZ1
Gene ID (NCBI) 84654
RRIDAB_2195884
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SPZ1 antibody 18931-1-APDownload protocol
IHC protocol for SPZ1 antibody 18931-1-APDownload protocol
IF protocol for SPZ1 antibody 18931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols