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SRFBP1 Polyclonal antibody

SRFBP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12792-1-AP

Synonyms

BUD22, P49, p49/STRAP, Rlb1, SRFBP1, STRAP

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using SRFBP1 Polyclonal antibody (12792-1-AP)

    WB analysis of mouse heart using 12792-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12792-1-AP (SRFBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using SRFBP1 Polyclonal antibody (12792-1-AP)

    WB analysis of HeLa using 12792-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12792-1-AP (SRFBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using SRFBP1 Polyclonal antibody (12792-1-AP)

    WB analysis of HepG2 using 12792-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12792-1-AP (SRFBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SRFBP1 Polyclonal antibody (12792-1-AP)

    IHC staining of human pancreas cancer using 12792-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12792-1-AP (SRFBP1 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inmouse heart tissue, HeLa cells, HepG2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12792-1-AP targets SRFBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SRFBP1 fusion protein Ag3517
Full Name serum response factor binding protein 1
Calculated Molecular Weight 429 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC031222
Gene Symbol SRFBP1
Gene ID (NCBI) 153443
RRIDAB_2877882
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SRFBP1, also named as P49, p49/STRAP and STRAP, is abundantly expressed in heart and skeletal muscle, and at much lower levels in brain and lung. It may be involved in regulating transcriptional activation of cardiac genes during the aging process. SRFBP1 may play a role in biosynthesis and/or processing of SLC2A4 in adipose cells. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human SRFBP1.

Protocols

Product Specific Protocols
WB protocol for SRFBP1 antibody 12792-1-APDownload protocol
IHC protocol for SRFBP1 antibody 12792-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols