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- KD/KO Validated
TRAPB/SSR2 Polyclonal antibody
TRAPB/SSR2 Polyclonal Antibody for WB, IF, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, FC, IHC, ELISA
Conjugate
Unconjugated
Cat no : 10278-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human breast cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 7 publications below |
| IHC | See 2 publications below |
| IP | See 1 publications below |
Product Information
10278-1-AP targets TRAPB/SSR2 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRAPB/SSR2 fusion protein Ag0304 |
| Full Name | signal sequence receptor, beta (translocon-associated protein beta) |
| Calculated Molecular Weight | 20 kDa |
| Observed Molecular Weight | 22-25 kDa |
| GenBank Accession Number | BC000341 |
| Gene Symbol | SSR2 |
| Gene ID (NCBI) | 6746 |
| RRID | AB_2195624 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TRAPB (translocon-associated protein subunit beta), also known as SSR2 (signal sequence receptor subunit beta), is a 22-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 7789174). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRAPB/SSR2 antibody 10278-1-AP | Download protocol |
| IHC protocol for TRAPB/SSR2 antibody 10278-1-AP | Download protocol |
| IF protocol for TRAPB/SSR2 antibody 10278-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins. | ||
Sci Adv The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress. | ||
Diabetes The Role of TRAPγ/SSR3 in Preproinsulin Translocation into the Endoplasmic Reticulum. | ||
FASEB J Deficient endoplasmic reticulum translocon-associated protein complex limits the biosynthesis of proinsulin and insulin.
| ||
J Cell Mol Med Overexpression of SSR2 promotes proliferation of liver cancer cells and predicts prognosis of patients with hepatocellular carcinoma.
| ||
J Immunol Res Endoplasmic Reticulum Stress-Related Signature for Predicting Prognosis and Immune Features in Hepatocellular Carcinoma |
