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SSR4 Polyclonal antibody

SSR4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11655-2-AP

Synonyms

SSR delta, SSR4, TRAP delta, TRAPD

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SSR4 Polyclonal antibody (11655-2-AP)

    WB analysis using 11655-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11655-2-AP (SSR4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using SSR4 Polyclonal antibody (11655-2-AP)

    WB analysis of human placenta using 11655-2-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11655-2-AP (SSR4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using SSR4 Polyclonal antibody (11655-2-AP)

    IP experiment of HeLa using 11655-2-AP

    IP result of anti-SSR4 (IP:11655-2-AP, 4ug; Detection:11655-2-AP 1:300) with HeLa cells lysate 2600 ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SSR4 Polyclonal antibody (11655-2-AP)

    IHC staining of human pancreas cancer using 11655-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11655-2-AP (SSR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using SSR4 Polyclonal antibody (11655-2-AP)

    IHC staining of human pancreas cancer using 11655-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11655-2-AP (SSR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SSR4 Polyclonal antibody (11655-2-AP)

    IF Staining of HeLa using 11655-2-AP

    Immunofluorescent analysis of HeLa cells using 11655-2-AP (SSR4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, human placenta tissue, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11655-2-AP targets SSR4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SSR4 fusion protein Ag2250
Full Name signal sequence receptor, delta (translocon-associated protein delta)
Calculated Molecular Weight 173 aa, 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC003371
Gene Symbol SSR4
Gene ID (NCBI) 6748
RRIDAB_2195738
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signal sequence receptor subunit delta (SSR4, or TRAPD) gene encodes the translocon-associated proteins subunit delta, which is about 19-kDa and is located at endoplasmic reticulum (ER) membrane, the translocon-associated proteins complex functions to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. TRAP-delta can form heterotetramer with other TRAP homolog TRAP-alpha, TRAP-beta and TRAP-gamma.

Protocols

Product Specific Protocols
WB protocol for SSR4 antibody 11655-2-APDownload protocol
IHC protocol for SSR4 antibody 11655-2-APDownload protocol
IF protocol for SSR4 antibody 11655-2-APDownload protocol
IP protocol for SSR4 antibody 11655-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress.

Authors - Chatchai Phoomak
View Article
humanIHC

Expert Rev Mol Diagn

SSR4 as a prognostic biomarker and related with immune infiltration cells in colon adenocarcinoma.

Authors - Weiwei He
View Article