• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

STARD3NL Polyclonal antibody

STARD3NL Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20502-1-AP

Synonyms

MENTHO, STARD3 N terminal like, STARD3 N terminal like protein, STARD3NL

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using STARD3NL Polyclonal antibody (20502-1-AP)

    WB analysis using 20502-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20502-1-AP (STARD3NL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using STARD3NL Polyclonal antibody (20502-1-AP)

    IP experiment of HEK-293 using 20502-1-AP

    IP result of anti-STARD3NL (IP:20502-1-AP, 4ug; Detection:20502-1-AP 1:500) with HEK-293 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using STARD3NL Polyclonal antibody (20502-1-AP)

    IHC staining of human breast cancer using 20502-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 20502-1-AP (STARD3NL antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using STARD3NL Polyclonal antibody (20502-1-AP)

    IHC staining of human breast cancer using 20502-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 20502-1-AP (STARD3NL antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using STARD3NL Polyclonal antibody (20502-1-AP)

    IF Staining of HEK-293 using 20502-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 20502-1-AP (STARD3NL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inU2OS cells, HEK-293 cells, mouse kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20502-1-AP targets STARD3NL in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STARD3NL fusion protein Ag14273
Full Name STARD3 N-terminal like
Calculated Molecular Weight 234 aa, 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC005959
Gene Symbol STARD3NL
Gene ID (NCBI) 83930
RRIDAB_10694281
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for STARD3NL antibody 20502-1-APDownload protocol
IHC protocol for STARD3NL antibody 20502-1-APDownload protocol
IF protocol for STARD3NL antibody 20502-1-APDownload protocol
IP protocol for STARD3NL antibody 20502-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

J Cell Mol Med

STARD3NL inhibits the osteogenic differentiation by inactivating the Wnt/β-catenin pathway via binding to Annexin A2 in osteoporosis.

Authors - Yuexin Xu
  • KD Validated
View Article